I like how Queen re-invents themselves with this album. The songs on this album are all catchy and sound great. I find myself sometimes singing several of these songs in my head.
I know that I wore the tape out when I had it then and will do the same with the CD. It is so dirty and kind of sexy at the same time. I love the horn arrangement.
Especially, the band break that just has the snapping of fingers. I can just picture "West Side Story. This is a very blatant anti-gun song. I sure not one of the NRA's favs. This is great song that is always being played on the local Classic Rock stations. I also like the cover art. It stands out and I know that is not something that one takes in account when buying a CD but I think is does look great.
Overall, I love this album. I consider it one of my "Classic Rock" albums to go along with my other Queen albums. It is a great buy and I think you will find yourself listening to it over and over again. See all reviews. Top reviews from other countries. If, like me, have the original vinyl pressings from the original date of release then you will prefer the sound quality from them rather than this collection.
OK, it's not typical Queen. OK, it's not aged well. BUT have those who dismissed this album truly explored all it has to offer? The lyrics and delivery of "Life is Real" by Freddie could easilly slot into any other Queen album and "Las Palabras de Amor" is my all time personal favourite Queen song that deserved to do better in the charts.
The rest of the album has a mix of songs that were a major departure but gave Queen the space to experiment with sounds that would later come together much stronger in "The Works" for example. Oh, and what about "Under Pressure"??!! Very good, Antibody (5) - Space Qveen (Cassette).
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Amazon Advertising Find, attract, and engage customers. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals. I used human breast cancer cell line MCF-7 cells. Negative control: cells only, secondary anti rabbit IgG fitc only. No positive control. I used up the antibody for real experiments. FITC secondary antibody was newly ordered, it is working very well in our lab's experiments.
I did not try to directly conjugated a tag to the antibody. How to do that? I put 10ul of you shipped antibody per test. I tried 5 ul to 10 ul of the antibody. Never works. I did not find the information in the data sheet. My cells should be live cells. Your antibody targets the N terminal of xCT protein. I searched xCT's structure, N Antibody (5) - Space Qveen (Cassette) locates intracellularly. Does that mean I could not use the antibody to target cell surface expression of xCT protein?
It seems only your company carry xCT ab for flow. I am quite desperate to get it to work! Thank you for your response and for the additional details which you have provided. Hi There, Our lab recently purchased xCT antibody ab for flow cytometry study. I want to measure xCT cell surface expression. However, I could never get a signal by flow.
I am wondering what's wrong. Can you give me some suggestions? Thank you for contacting Abcam, I am sorry to Antibody (5) - Space Qveen (Cassette) that you have been experiencing difficulties using this product in flow. Would you be able to provide me the specific details of your experiments? Useful information would include but not be limited to: Cell type, species and any treatments.
Isotype controls used. Positive controls. Have you used the secondary antibody with luck in flow? Have you attempted to directly conjugate a tag to the antibody? How many cells were used per experiment? What was the concentration of your lot and overall concentration of the antibody in your cell solution?
Have you attempted different concentrations? Any data is also very helpful. I would also suggest reviewingPampliega O et al.
This product is covered by our Abpromise guarantee. If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, as you are using it according to specifications listed on our datasheet. I'm wondering if you could send me a aliquot of your xCT antibody ab Indeed, I would like to test it on fresh samplesthat will beready at the end of the monthand, at the moment, we are not able to make any order indeed we are changing oursystemof prurchase.
At the end of the month however, everything will be OK and we will be able to order again. I could then regularize the order if you are agree. Let me Antibody (5) - Space Qveen (Cassette) your decision, hoping you will be able to Antibody (5) - Space Qveen (Cassette) mean aliquotas soon as possible. Thank you for contacting Abcam. When your ordering system is reorganized at the end of the month, you will be able to order the antibody and if it is in stock then it will be shipped overnight for next day delivery.
Therefore you will still be able to use the antibody on your fresh samples. Please let me know if there is anything else I can help you with. Multiple bands in addition to specific band,using human primary blood samples. Antibody (5) - Space Qveen (Cassette) you for your call today and for letting us know about the trouble with ab Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines.
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